Analysis Note

Positive ControlHePG2 cells, human placenta tissue

Application

ELISA (see comments)Immunoblotting (1-5 g/ml)Paraffin Sections (3 g/ml)

General description

This Anti-VCP Mouse mAb (4A8) is validated for use in ELISA, Immunoblotting, Paraffin Sections for the detection of VCP.

Recognizes the ~67 kDa VCP protein in HePG2 cells and human placenta tissue.

Purified mouse monoclonal antibody. Recognizes the ~67 kDa VCP protein.

Immunogen

Full-length, recombinant human VCP (aa 1-589), expressed as a GST fusion protein

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Regulatory Review (Z)